UCP1 Polyclonal Antibody-BS70689

UCP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70689-50, BS70689-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25874

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF: 1:50~1:100

Background: Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis. Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein. Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding UCP1 gene. Related studies link UCP1 to the possible development of obesity and type 2 diabetes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: UCP1 polyclonal antibody detects endogenous levels of UCP1 protein.

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using UCP1 antibody at 1:710 dilution., Immunohistochemistry of paraffin-embedded mouse brown adipose using UCP1 pAb at dilution of 1:50 (40x lens)., Immunohistochemistry of paraffin-embedded rat brown adipose using UCP1 pAb at dilution of 1:50 (40x lens)., Immunofluorescence analysis of mouse brown adipose cells using UCP1 pAb at dilution of 1:50 (40x lens).

Alternative Name: UCP1; SLC25A7; UCP

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human UCP1 (NP_068605.1).

Conjugate: Unconjugated

Modification: Unmodified