UCP3 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS80119-50, BS80119-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P55916 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Bioworld Molecular Weight: 34kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using UCP3 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per Lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s., Immunofluorescence analysis of C6 cells using UCP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using UCP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using UCP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: UCP3; SLC25A9 Immunogen: Recombinant fusion protein of human UCP3(P55916). Conjugate: Unconjugated Modification: Unmodified