Uroplakin 3a Monoclonal Antibody-MB66034

Uroplakin 3a Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66034-50, MB66034-100 Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: O75631 Host: Mouse Reactivity: Human Applications: IHC All Applications: IHC (1/100 - 1/300) Background: The asymmetric unit membrane (AUM) forms numerous plaques, which cover the apical surface of the urothelium. These plaques are thought to strengthen the urothelium and reduce the risk of rupturing during bladder distention. They are composed of four major integral membrane proteins called uroplakins (UP). The uroplakin family comprises UPIa, UPIb, UPII and UPIII. Family members are conserved among several species, including human, mouse, rat, rabbit, canine, porcine and ovine. UPIa and UPIb form tightly packed structures with UPII and UPIII, respectively. This pairing is required for normal urothelial plaque formation and is regulated by proteolytic processing of the uroplakin proteins. Uroplakins are expressed in normal urothelium and are used as specific markers of urothelial differentiation. They are also expressed in a majority of transitional cell carcinomas of the bladder (TCCs), which make the uroplakins a useful marker for detecting bladder cancer metastasis and for staging and monitoring chemotherapeutic response. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of Uroplakin 3a staining in human bladder formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Bioworld Molecular Weight: ~ 30 kDa Specificity: Recognizes endogenous levels of Uroplakin 3a protein. Alternative Name: UPK3; Uroplakin-3a; UP3a; Uroplakin III; UPIII Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Uroplakin 3a. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified