Product Description

V5-tag Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB8023-50, MB8023-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Mouse

Reactivity: Transfected

Applications: WB, IP,

All Applications: WB: 1:5000~1:20000
IP：1:100~1:1000

Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: V5 mAb detects endogenous levels of V5 protein.

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of V5 mAb at 1:5000 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (10ug)
Lane 3: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (20ug)
Lane 4: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (30ug)
Lane 5: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (40ug), Immunoprecipitation of HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate using V5 mAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4). Lane 1 is 20% input. The western blot was probed using V5 mAb #MB8023. “↑”（supernatant); “↓”（deposition）

Alternative Name: V5tag

Immunogen: Synthetic peptide, corresponding to V5 tag.

Conjugate: Unconjugated

Modification: Unmodified