VEGF Polyclonal Antibody - E-AB-93319

VEGF Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-93319-60, E-AB-93319-120, E-AB-93319-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: VEGF

Target Synonym: VEGFA; MVCD1; VEGF; VPF; vascular endothelial growth factor A

Research Areas: Cancer, Cardiovascular, Metabolism, Developmental Biology, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P15692

Background: This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

Concentration: 1mg/mL

Immunogen: A synthetic peptide of human VEGF

Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IF 1:50-1:200

Calculated MW: 15-27kDa/34-45kDa

Observed MW: 17KDa/45KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only