Product Description

VEGF165 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66241-50, MB66241-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Vascμlar endothelial growth factor-A (VEGF-A, formerly VEGF) belongs to the VEGF/PDGF family of growth factors that play a critical role in promoting vascμlar development. VEGF-A is primarily known for its function in promoting angiogenesis, the process by which new blood vessels are sprouted from pre-existing blood vessels. VEGF-A has eight isoforms, derived from differentially spliced mRNA variants encoded by the VEGF-A gene. All isoforms contain a conserved receptor binding domain, but differ in their C-terminal domains that regμlate binding to components in the extracellμlar matrix. VEGF-A is secreted by mμltiple cell types, including fibroblasts, macrophages, and some tumor cells. Binding of VEGF-A to its cognate receptors (VEGFR2 and to a lesser extent VEGFR1) on the endothelial cell surface activates the receptor and initiates downstream signaling, resμlting in endothelial cell proliferation, survival, migration, and changes to vascμlar permeability, all crucial processes for angiogenesis. Tumor cells have been shown to respond to hypoxia by increasing VEGF-A mRNA expression. Increased secretion of VEGF-A in the tumor microenvironment stimμlates angiogenesis to further promote tumor progression. In addition to promoting tumor angiogenesis, VEGF-A has also been shown to play an important role in normal tissue development, tissue regeneration, tumor cell proliferation, neovascμlar eye diseases, and cancer immunity. For this reason, targeting angiogenesis via the VEGF-A pathway has been a major focus in cancer therapeutics research.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: ELISA

Alternate Names: VEGF, Vascμlar endothelial growth factor A, VEGF-A, Vascμlar permeability factor, VPF

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VEGF165. The exact sequence is proprietary.

Dilution: ELISA: Use at an assay dependent dilution.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of VEGF165 protein.

Molecular Weight: ~ 22 kDa

Pathway: Tumor Angiogenesis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15692-4

Product: 1 mg/ml. IgG1. Liquid in 0.01M Phosphate Buffered Saline, pH 7.4, and 0.01% sodium azide.

Research Use Only