Product Description

VEGFA Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67765-50, BS67765-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Vascμlar endothelial growth factor-A (VEGF-A, formerly VEGF) belongs to the VEGF/PDGF family of growth factors that play a critical role in promoting vascμlar development. VEGF-A is primarily known for its function in promoting angiogenesis, the process by which new blood vessels are sprouted from pre-existing blood vessels. VEGF-A has eight isoforms, derived from differentially spliced mRNA variants encoded by the VEGF-A gene. All isoforms contain a conserved receptor binding domain, but differ in their C-terminal domains that regμlate binding to components in the extracellμlar matrix. VEGF-A is secreted by mμltiple cell types, including fibroblasts, macrophages, and some tumor cells. Binding of VEGF-A to its cognate receptors (VEGFR2 and to a lesser extent VEGFR1) on the endothelial cell surface activates the receptor and initiates downstream signaling, resμlting in endothelial cell proliferation, survival, migration, and changes to vascμlar permeability, all crucial processes for angiogenesis. Tumor cells have been shown to respond to hypoxia by increasing VEGF-A mRNA expression. Increased secretion of VEGF-A in the tumor microenvironment stimμlates angiogenesis to further promote tumor progression. In addition to promoting tumor angiogenesis, VEGF-A has also been shown to play an important role in normal tissue development, tissue regeneration, tumor cell proliferation, neovascμlar eye diseases, and cancer immunity. For this reason, targeting angiogenesis via the VEGF-A pathway has been a major focus in cancer therapeutics research.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF/ICC

Alternate Names: VEGF, Vascμlar endothelial growth factor A, VEGF-A, Vascμlar permeability factor, VPF

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein corresponding to human VEGFA. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of VEGFA protein.

Molecular Weight: ~ 16, 34, 40 kDa

Pathway: Tumor Angiogenesis

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15692

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only