Vimentin Monoclonal Antibody - BT-MCA3346

Vimentin Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Number: BT-MCA3346-50, BT-MCA3346-100

Citations, Manuals and MSDS Available upon request.

Background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Research Areas: Neuroscience

Target Protein: Vimentin

Applications: WB, IHC

Reactivity: Human

Clonality: Monoclonal

Clone ID: 9E7E7

Host: Mouse

Isotype: Mouse IgG1

Full Product Name: Vimentin

Immunogen: Purified recombinant fragment of Vimentin expressed in E. Coli.

Storage: 4°C|-20°C for long term storage

Formulation: Ascitic fluid containing 0.03% sodium azide.

UniProt Accession: P08670

Synonyms: FLJ36605, VIM

GeneID: 7431

Research Use Only