Product Description

WT1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS91456-50, BS91456-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10, 000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF, IHC, ELISA

Alternate Names: WIT 2, WT 1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1, Wilms Tumor, Wilms tumor protein, Wilms' tumor gene, Wilms' tumor protein, WIT2, WT, WT1, WT-1, WT1_HUMAN, WT33

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH conjugated synthetic peptide derived from human WT1: 301-400/449

Dilution: WB: 1:500~1:2000
IHC: 1:100~1:500
IF: 1:100~1:500
ELISA: 1:5000~1:10000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: WT1 polyclonal antibody detects endogenous levels of WT1 protein.

Molecular Weight: ~ 48 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P19544

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only