Product Description

WTAP (H351) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2062-50, BS2062-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Wilms’ tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10, 000 infants and is observed in both sporadic and inherited forms. The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regμlating mRNA splicing. WTAP is found in nuclear speckles, where it regμlates the G2/M cell cycle transition by binding to the 3’ UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: Pre-mRNA-splicing regμlator WTAP, Female-lethal(2)D homolog, hFL(2)D, WT1-associated protein, Wilms tumor 1-associating protein, WTAP, KIAA0105

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 320-374 of Human WTAP.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: WTAP (H351) polyclonal antibody detects endogenous levels of WTAP protein.

Molecular Weight: ~ 44 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15007

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only