Product Description

XPA (H244) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1492-50, BS1492-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23025

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: The XPA gene encodes a 31 kDa zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair. The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (XPV) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of XPA (H244) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50. showing Nucleus staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 31, 39 kDa

Specificity: XPA (H244) polyclonal antibody detects endogenous levels of XPA protein.

Alternative Name: DNA repair protein complementing XP-A cells; Xeroderma pigmentosum group A-complementing protein; XPA; XPAC

Immunogen: Synthetic peptide, corresponding to amino acids 215-260 of Human XPA.

Conjugate: Unconjugated

Modification: Unmodified