Product Description

YAP (phospho-Ser109) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES12270-50, ES12270-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Yorkie homolog (65 kDa Yes-associated protein) (YAP65)

Clonality: Polyclonal

Immunogen: Synthesized phosho peptide around human YAP (Ser109)

Isotype: IgG

Target: YAP,

Specificity: This antibody detects endogenous levels of Human Mouse Rat YAP (phospho-Ser109)

Dilution: WB 1:1000-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

Observed Band (KD): 67kD

Fields: Hippo signaling pathway, Hippo signaling pathway - multiple species

Function: PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 WW domain. Subunit: Binds to the SH3 domain of the YES kinase. Binds to WBP1 and WBP2. Binds, in vitro, through the WW1 domain, to neural isoforms of ENAH that contain the PPSY motif.

Subcellular Location: Cytoplasm Nucleus Both phosphorylation and cell density can regulate its subcellular localization (PubMed:18158288, PubMed:20048001). Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus (PubMed:18158288, PubMed:20048001). At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001, PubMed:25849865). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity).

Storage and Stability: -20°C/1 year

Gene Name: YAP1, YAP65

Protein Name: YAP, Ser109

Human Gene ID: 10413

Human SwissProt Number: P46937

Mouse Gene ID: 22601

Mouse SwissProt Number: P46938

Rat Gene ID: 363014

Rat SwissProt Number: Q2EJA0

Research Use Only