Product Description

ZAP70 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66578-50, MB66578-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia. Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimμlated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IP

Alternate Names: SRK, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human ZAP-70 protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of ZAP70 protein.

Molecular Weight: ~ 70 kDa

Pathway: ErbB HER Signaling，PI3K AKT signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P43403

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only