Product Description

ZAP70 (phospho-Y315) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4884-50, BS4884-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a mμltisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding resμlts in the phosphorylation of ZAP-70 on mμltiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regμlate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regμlated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 315.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-ZAP70 (Y315) polyclonal antibody detects endogenous levels of ZAP70 protein only when phosphorylated at Tyr315.

Molecular Weight: ~ 70 kDa

Pathway: Immune Cell Signaling, T cell receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P43403

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only