Product Description

ZIS (I144) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1900-50, BS1900-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95218

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF265 (Zinc finger protein 265), also known as ZRANB2 (Zinc finger Ran-binding domain-containing protein 2), ZIS, ZIS1 or ZIS2, is a 330 amino acid protein that belongs to the ZRANB2 family. Localized to the nucleus, ZNF265 functions as a splicing factor that is responsible for alternatively splicing Tra-2β (transformer-2 beta) transcripts and is thought to interfere with constitutive 5’-splice selection. ZNF265 contains two RanBP2-type zinc fingers through which it conveys its RNA-binding activity. Two isoforms, designated ZIS-1 and ZIS-2, are expressed due to alternative splicing events. Upon DNA damage, ZIS-2 may be phosphorylated by ATM or ATR.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of ZIS (I144) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 37 kDa

Specificity: ZIS (I144) polyclonal antibody detects endogenous levels of ZIS protein.

Alternative Name: Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger, splicing; ZRANB2; ZIS; ZNF265

Immunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human ZIS.

Conjugate: Unconjugated

Modification: Unmodified