Product Description

β-1,3-Glucanase Activity Colorimetric Assay Kit

Sizes: 96T

Catalogue Numbers: E-BC-K1223-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: β-1, 3-glucanase (β-1,3-GA, EC 3.2.1.39) is mainly found in plants and catalyzes
the hydrolysis of β-1, 3-glucosidic bonds, thereby destroying fungal cell walls. In
particular, when synergistic with chitinase, it can significantly inhibit the growth
of fungi. When plants are infected with diseases or under other stress conditions,
they can induce a large amount of β-1, 3-GA to enhance plant resistance to
adverse external stimuli. Therefore, the determination of β-1, 3-GA activity is
widely used in plant pathology and stress physiology research.
β-1,3-GA hydrolyses the β-1, 3-glucosidic linkage of laminarin to produce
reducing ends. The activity can be reflected by measuring the amount of reducing
sugar using 3, 5-dinitrosalicylic acid.

Research Areas: Plant Physiology Series

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 6.22-333.33 U/mL

Tested Sample Types: Plant tissue

Assay Length: 50 min

Sensitivity: 15.33 U/mL

Inter cv: 4.1-6.4%

Intra cv: 2.6-3.8%

Research Use Only