Product Description

β-actin Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: EA014-50, EA014-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: actin antibody, b-actin antibody, beta-actin antibody

Clonality: Polyclonal

Immunogen: Recombinant Protein

Isotype: IgG

Dilution: WB 1:3,000 IHC 1:200 IF 1:200

Concentration: 1mg/mL,

Observed Band (KD): 43kDa

Subcellular Location: Cytoplasm, Cytoskeleton

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Research Use Only