Product Description

γ-Catenin Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6066-50, BS6066-100

Swiss-Prot: P14923

Host: Rabbit

γ-Catenin Polyclonal Antibody-BS6066

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 82kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using γ-Catenin antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit., Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug γ-Catenin antibody. Western blot was performed from the immunoprecipitate using γ-Catenin antibody at a dilution of 1:1000.

Alternative Name: JUP; ARVD12; CTNNG; DP3; DPIII; PDGB; PKGB

Immunogen: Recombinant fusion protein of human γ-Catenin(NP_002221.1).

Modification: Unmodified

Conjugate: Unconjugated