Product Description

1,5-AG (1,5-Anhydroglucitol) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7840-48, ELK7840-96, ELK7840-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: LycoMark, 1-Deoxy-D-glucose, 1-Deoxy-D-glucopyranose, 1, 5-Anhydro-D-glucitol, 1, 5-Anhydrosorbitol, Aceritol, Polygalytol

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Enzyme and Kinase, Metabolic Pathway, Endocrinology, Nutrition Metabolism

Assay Length: 2h

Sensitivity: 0.6 µg/mL

Standard: 150 µg/mL

Detection Range: 2.35-150 µg/mL

Research Use Only