1,5-AG (1,5-Anhydroglucitol) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7840-48, ELK7840-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.6 µg/mL
Standard: 150 µg/mL
Detection Range: 2.35-150 µg/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Enzyme and Kinase; Metabolic pathway; Endocrinology; Nutrition metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.