Product Description

12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8156-48, ELK8156-96, ELK8156-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 12HETE

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Signal Transduction, Metabolic Pathway, Infection Immunity, Hormone Metabolism, Dermatology

Assay Length: 2h

Sensitivity: 5.13 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Research Use Only