Product Description

3-Hydroxy-3-methylglutaryl-CoA Reductase (HMGR) Inhibitor Screening Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-D011-48, E-BC-D011-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is the rate-limiting enzyme of mevalonate pathway, which is the metabolic pathway of acetyl-CoA to produce cholesterol.This detection kit can screen inhibitors of HMGR, which is an important tool for basic research of cholesterol and other related metabolic pathways.
The detection principle of this kit: HMGR catalyzed substrate reaction consumes NADPH, and the absorbance decreases at 340 nm. The addition of inhibitors will inhibit HMGR enzyme activity, resulting in a decrease in the rate of absorbance decline. The inhibition rate can be calculated by measuring the change value of absorbance.

Abbreviation: HMGR

Research Areas: Glycolysis and Lipid Metabolism;Inhibitor Screening

Result Type: Inhibition rate

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Tested Sample Types: Inhibitor

Assay Length: 40 min

Inter cv: 2.2-10.0%

Intra cv: 1.8-3.3%

Research Use Only