3-NT (3-Nitrotyrosine) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7866-48, ELK7866-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.35 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Metabolic pathway; Infection immunity; Rheumatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.