Product Description

4-HNE (4-Hydroxynonenal) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9581-48, ELK9581-96, ELK9581-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal

Reactivity: General

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Signal Transduction, Metabolic Pathway, Infection Immunity, Hormone Metabolism, Dermatology

Assay Length: 2h

Sensitivity: 0.38 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Research Use Only