4-HNE (4-Hydroxynonenal) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9581-48, ELK9581-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.38 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 2h
Research Areas: Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.