Product Description

5-Me-THF (5-Methyltetrahydrofolate) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8059-48, ELK8059-96, ELK8059-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 5-Me-THFA, 5-Me-H4FA, 5-Me-THF, 5-Me-H4F, Levomefolic Acid, Metafolin, 5-Methyltetrahydrofolic Acid, L-Methylfolate

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Hematology, Reproductive Science, Nutrition Metabolism

Assay Length: 2h

Sensitivity: 0.44 ng/mL

Standard: 90 ng/mL

Detection Range: 1.41-90 ng/mL

Research Use Only