Product Description

53BP1 (phospho-Ser1778) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES18530-50, ES18530-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB

Alternate Names: Tumor suppressor p53-binding protein 1 (53BP1) (p53-binding protein 1) (p53BP1)

Clonality: Polyclonal

Immunogen: Synthesized phosho peptide around human 53BP1 (Ser1778)

Isotype: IgG

Target: 53BP1

Specificity: This antibody detects endogenous levels of Human 53BP1 (phospho-Ser1778)

Dilution: WB 1:1000-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Cerebellum, Cervix, Epithelium, Myeloid leukemia cell, Skeletal muscle,

Observed Band (KD): 213kD

Fields: NOD-like receptor signaling pathway

Function: Function: May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage
PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding
PTM: Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation
Similarity: Contains 2 BRCT domains
Subcellular Location: Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks
Subunit: Interacts with IFI202A (By similarity). Binds to th

Subcellular Location: Nucleus Chromosome Chromosome, centromere, kinetochore Localizes to the nucleus in absence of DNA damage (PubMed:28241136) Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs): recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600) Associated with kinetochores during mitosis (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: TP53BP1

Protein Name: 53BP1 (Ser1778)

Mouse Gene ID: 27223

Mouse SwissProt Number: P70399

Research Use Only