53BP1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-68380-60, E-AB-68380-120, E-AB-68380-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: 53BP1
Target Synonym: TP53BP1; 53BP1; TDRD30; TP53; p202; p53BP1
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q12888
Background: Double-strand break (DSB repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs in response to DNA damage by promoting non-homologous end joining (NHEJ-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub and histone H4 dimethylated at 'Lys-20' (H4K20me2, two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity. In contrast, it is not required for classic NHEJ and V(DJ recombination (By similarity. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Concentration: 1 mg/mL
Immunogen: Recombinant fusion protein of human 53BP1
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:50-1:200
Calculated MW: 213kDa/214kDa
Observed MW: 450KDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only