Product Description

8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10848-48, ELK10848-96, ELK10848-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 8OHdG, 7, 8-Dihydro-8-Oxo-2'-Deoxyguanosine, 7, 8-Dihydro-8-Oxodeoxyguanosine, 8-Hydroxy-2'-Deoxyguanosine, 8-Oxo-DG, 7, 8-dihydrodeoxyguanosine

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolic Pathway, Tumor Immunity, Infection Immunity, Endocrinology, Hematology, Hepatology

Assay Length: 2h

Sensitivity: 0.57 ng/mL

Standard: 100 ng/mL

Detection Range: 1.56-100 ng/mL

Research Use Only