ABflo® 488 Rabbit anti-Human CD146 Monoclonal Antibody
Sizes: 100T, 2 x 100T, 5 x 100T
Catalogue Numbers: A22520-100, A22520-200, A22520-500
Citations, Manuals and MSDS Available upon request.
Background: Melanoma cell adhesion molecule (MCAM, MUC18, CD146) is an immunoglobulin superfamily member originally described as a cell surface adhesion protein and marker of the progression and metastasis of melanoma . Expression of MCAM protein is seen in vascular endothelial cells, activated T lymphocytes, smooth muscle, and bone marrow stromal cells. Research studies demonstrate increased MCAM expression in endothelial cells from angiogenesis-related disorders, including inflammatory bowel disease, Crohn’s disease, rheumatoid arthritis, tumors, and chronic renal failure . MCAM-expressing human mesenchymal stromal cells (hMSC) in the hematopoietic microenvironment are responsible for maintaining the self-renewal of hematopoietic stem and progenitor cells (HSPC) through direct contact between hMSC and HSPC. Related studies suggest that activation of the Notch signaling pathway may also, in part, play a role in HSPC maintenance . Additional research indicates that MCAM may play a role in multiple sclerosis, an autoimmune inflammatory disease that affects central nervous system neurons. Endothelial MCAM within the blood-brain barrier act as adhesion receptors that permit lymphocytes to transmigrate across the barrier and produce the inflammatory lesions that characterize the disorder .
Category: Monoclonal Antibodies
Application: FC
Cross Reactivity: Human
Protein Weight: 72kDa Immunogen: Recombinant protein (or fragment).
Species: Human
GeneID: 4162
SWISS: P43121
Alternate Names: CD146; MUC18; HEMCAM; METCAM; MelCAM
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at 2-8℃. Avoid freeze.|Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilution: FC,5 μl per 10^6 cells in 100 μl volume
Research Areas: Cancer, Invasion and Metastasis, Signal Transduction, Cell Biology Developmental Biology, Cell Adhesion, Cytoskeleton, Immunology Inflammation, CDs, Stem Cells
NCBI Alias: MCAM
Modifications: Unmodified
Conjugate: ABflo® 488. Ex:491nm. Em:516nm.
Clone Number Info: ARC56715
Research Use Only