ABflo® 594 Rabbit anti-Human/Monkey IgM Monoclonal Antibody
Sizes: 100T, 2 x 100T, 5 x 100T
Catalogue Numbers: A24696-100, A24696-200, A24696-500
Citations, Manuals and MSDS Available upon request.
Background: Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Category: Monoclonal Antibodies
Application: IF/ICC, FC
Cross Reactivity: Human, Cynomolgus
Protein Weight: 49kDa Immunogen: Recombinant protein (or fragment).
Species: Human
GeneID: 3507
SWISS: P01871-1
Alternate Names: IGHM; AGM1; MU; VH; immunoglobulin heavy constant mu
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at 2-8℃. Avoid freeze.|Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilution: IF/ICC,1:50-1:200|FC,5 μl per 10^6 cells in 100 μl volume
Research Areas: Immunology and Inflammation
NCBI Alias: IGHM
Modifications: Unmodified
Conjugate: ABflo® 594. Ex:588nm. Em:604nm.
Clone Number Info: ARC63173
Research Use Only