Product Description

ACACB Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES18505-50, ES18505-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human ACACB

Isotype: IgG

Target: ACACB

Specificity: This antibody detects endogenous levels of ACACB at Human

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716), Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759), Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759),

Molecular Weight (Da): 270kD

Fields: Fatty acid biosynthesis, Pyruvate metabolism, Propanoate metabolism, Metabolic pathways, AMPK signaling pathway, Insulin signaling pathway, Adipocytokine signaling pathway, Glucagon signaling pathway, Insulin resistance, Alcoholic liver disease

Function: Catalytic Activity: ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
Catalytic Activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein
Cofactor: Binds 2 manganese ions per subunit
Cofactor: Biotin
Enzyme Regulation: Activated by citrate. Inhibited by malonyl-CoA
Function: ACC-beta may be involved in the provision of malonyl-CoA or in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase
Pathway: Lipid metabolism, malonyl-CoA biosynthesis, malonyl-CoA from acetyl-CoA: step 1/1
Similarity: Contains 1 ATP-grasp domain
Similarity: Contains 1 biotin carboxylation domain
Similarity: Contains 1 biotinyl-binding domain
Similarity: Contains 1 carboxyltransferase domain
Subcellular Location: May associa

Subcellular Location: Mitochondrion

Storage and Stability: -20°C/1 year

Gene Name: ACACB ACC2 ACCB

Protein Name: ACACB

Research Use Only