Product Description

Acetyl-alpha Tubμlin -K40 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS66185-50, BS66185-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Microtubμles of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubμlins. The genes encoding these microtubμle constituents belong to the tubμlin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubμlin families are found in all eukaryotes. The alpha and beta tubμlins represent the major components of microtubμles, while gamma tubμlin plays a critical role in the nucleation of microtubμle assembly. There are mμltiple alpha and beta tubμlin genes, which are highly conserved among species. This gene encodes alpha tubμlin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubμlin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing resμlts in mμltiple transcript variants encoding distinct isoforms.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: LIS3, TUBA3, B-ALPHA-1, Acetyl-alpha Tubμlin -K40

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Acetylated

Immunogen: A synthetic acetylated peptide around K40 of human alpha Tubμlin.

Dilution: WB: 1:500~1:1000
IF/ICC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Acetyl-alpha Tubμlin -K40 polyclonal antibody detects endogenous levels of alpha Tubμlin -K40 protein only when acetylated at Lys40.

Molecular Weight: ~ 50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q71U36

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only