Product Description

Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F046-48, E-BC-F046-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle.
The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.

Abbreviation: Acetyl-CoA

Research Areas: Tricarboxylic Acid（TCA）Cycle;Energy Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 0.27-5.00 μmol/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 30 min

Sensitivity: 0.27 μmol/L

Inter cv: 2.7-3.6%

Intra cv: 1.7-4.1%

Research Use Only