Acetyl-Histone H3-K27 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A7253-20, A7253-100
Citations, Manuals and MSDS Available upon request.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Category: Acetylated Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA, ChIP, ChIP-seq
Cross-reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Protein Weight: 15kDa
Observed Molecular Weight: 17kDa
Immunogen: Synthetic peptide .
Species: Human
GeneID: 8290/8350
SWISS: Q16695/P68431
Alternate Names: H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K27
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:1000 - 1:5000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP, 5μg antibody for 5μg-10μg of Chromatin ChIP-seq, 1:20 - 1:100
Research Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway.
NCBI Alias: H3-4
Research Use Only