Adducin β (E497) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS2523-50, BS2523-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35612
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
Background: Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Adducin β (E497) polyclonal antibody detects endogenous levels of Adducin β protein.
Bioworld Molecular Weight: ~ 81 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Adducin β (E497) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: The Brain tissue lysate of Mouse (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: K562 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β
Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin β.
Conjugate: Unconjugated
Modification: Unmodified