Product Description

AF-4 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP00284-20, BT-AP00284-50, BT-AP00284-100

Citations, Manuals and MSDS Available upon request.

Background: AFF1 encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, AFF1has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.

Research Areas: Epigenetics and Nuclear Signaling

Target Protein: AFF1

Applications: IHC-p, WB, ELISA

Reactivity: Human, Mouse

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: AF-4 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 131422

UniProt Accession: Human: P51825; Mouse: O88573

Synonyms: AFF1; AF4; FEL; MLLT2; PBM1; AF4/FMR2 family member 1; ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4

GeneID: Human: 4299

Research Use Only