Product Description

Alanine (Ala) Colorimetric Assay Kit

Sizes: 96T

Catalogue Numbers: E-BC-K839-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Alanine (Ala) is a non-essential amino acid, which is one of the 20 basic amino acids that make up proteins. Ala has a high content in human blood and plays an important role in human metabolic activities such as glucose-alanine cycle. It can also help the nervous system conduction and plays an important role in maintaining human health.
The detection principle of this kit is as follows: alanine is decomposed by enzymes to generate NADH, which is catalyzed by electron carrier to form a chromogenic substance with WST-8. The chromogenic substance has a maximum absorption at the wavelength of 450 nm, and the Ala content in the sample is calculated by measuring the OD value at 450 nm and the standard curve.

Abbreviation: Ala

Research Areas: Amino Acids and Proteins

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 10.65-500 μmol/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 1 h 30 min

Sensitivity: 10.65 μmol/L

Inter cv: 4.8-8.4%

Intra cv: 2.2-2.8%

Research Use Only