Product Description

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K235-M-48, E-BC-K235-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Abbreviation: ALT

Target Synonym: ALT;GPT;Glutamic-pyruvic transaminase

Research Areas: Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.75-72.30 IU/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell;Cell culture supernatant

Assay Length: 1 h 20 min

Sensitivity: 0.75 IU/L

Inter cv: 9%

Intra cv: 5.3%

Research Use Only