Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit - E-BC-F038

Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F038-48, E-BC-F038-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: ALT

Research Areas: Liver and Renal Function

Result Type: Enzyme Activity

Detection Instrument: Fluorescence Microplate Reader

Tested Sample Types: Serum, plasma, animal tissue

Assay Time: 75 min

Detection Type: Fluorescence method

Sensitivity: 0.01 U/L

Detection Range: 0.01-0.83 U/L

Test Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Inter CV: 9.8

Intra CV: 2.3

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag