Product Description

Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F038-48, E-BC-F038-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Abbreviation: ALT

Target Synonym: ALT

Research Areas: Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 0.01-0.83 U/L

Tested Sample Types: Serum;Plasma;Animal tissue

Assay Length: 1 h 15 min

Sensitivity: 0.01 U/L

Inter cv: 9.8%

Intra cv: 2.3%

Research Use Only