Product Description

ALKB2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES9268-50, ES9268-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein. at AA range: 140-220

Isotype: IgG

Target: ALKB2

Specificity: ALKB2 Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder,

Observed Band (KD): 28kD

Function: Cofactor: Binds 1 Fe(2+) ion per subunit
Enzyme Regulation: Activated by ascorbate
Function: Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron
Similarity: Belongs to the alkB family
Subcellular Location: Detected in replication foci during S-phase
Tissue Specificity: Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder

Subcellular Location: Nucleus Nucleus, nucleolus Nucleus, nucleoplasm Relocates to the replication foci during S-phase

Storage and Stability: -20°C/1 year

Gene Name: ALKBH2 ABH2

Protein Name: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 (EC 1.14.11.33) (Alkylated DNA repair protein alkB homolog 2) (DNA oxidative demethylase ALKBH2) (Oxy DC1)

Mouse SwissProt Number: Q6P6J4

Research Use Only