Product Description

AMPKα1 (R493) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5552-50, BS5552-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AMPK is a heterotrimeric complex comprising a catalytic α subunit and regμlatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regμlation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regμlate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regμlatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, au-protein kinase PRKAA1, PRKAA1, AMPK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human AMPKα1.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AMPKα1 (R493) polyclonal antibody detects endogenous levels of AMPKα1 protein. The antibody does not cross-react with AMPKα2.

Molecular Weight: ~ 63 kDa

Pathway: Hypoxia Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Insμlin &Glucose Signaling, AMPK Signaling Pathway, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease, MTOR signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13131

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only