Product Description

Annexin I (phospho Tyr21) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1534-50, ES1534-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: ANXA1, ANX1, LPC1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35

Clonality: Polyclonal

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Annexin I (phospho Tyr21)

Isotype: IgG

Target: Annexin I

Specificity: Phospho-Annexin I (Y21) Polyclonal Antibody detects endogenous levels of Annexin I protein only when phosphorylated at Y21.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in resting neutrophils (PubMed:10772777), Detected in peripheral blood T-cells (PubMed:17008549), Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:25664854), Detected in placenta (at protein level) (PubMed:2532504), Detected in liver,

Molecular Weight (Da): 39kD

Function: Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid
Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity
PTM: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity
Similarity: Belongs to the annexin family
Similarity: Contains 1 annexin repeat
Similarity: Contains 2 annexin repeats
Similarity: Contains 4 annexin repeats
Subcellular Location: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages
Subunit: Homodimer in placenta (20%), linked by transglutamylat

Subcellular Location: Nucleus Cytoplasm Cell projection, cilium Cell membrane Membrane, Peripheral membrane protein Endosome membrane, Peripheral membrane protein Basolateral cell membrane Apical cell membrane Lateral cell membrane Secreted Secreted, extracellular space Cell membrane, Peripheral membrane protein, Extracellular side Secreted, extracellular exosome Cytoplasmic vesicle, secretory vesicle lumen Cell projection, phagocytic cup Early endosome Cytoplasmic vesicle membrane, Peripheral membrane protein Secreted, at least in part via exosomes and other secretory vesicles Detected in exosomes and other extracellular vesicles (PubMed:25664854) Alternatively, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10, it results in t

Storage and Stability: -20°C/1 year

Gene Name: ANXA1

Protein Name: Annexin A1

Mouse SwissProt Number: P10107

Research Use Only