Product Description

AP-1 (phospho Ser63) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1260-50, ES1260-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, WB, IHC, ELISA

Alternate Names: JUN, Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Ser63. AA range:31-80

Isotype: IgG

Target: c-JUN

Specificity: Phospho-AP-1 (S63) Polyclonal Antibody detects endogenous levels of AP-1 protein only when phosphorylated at S63.

Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in the developing and adult prostate and prostate cancer cells,

Observed Band (KD): 39-42kD

Fields: Endocrine resistance, MAPK signaling pathway, ErbB signaling pathway, cAMP signaling pathway, Mitophagy - animal, Apoptosis, Wnt signaling pathway, Osteoclast differentiation, Focal adhesion, Tight junction, Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, C-type lectin receptor signaling pathway, IL-17 signaling pathway, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, B cell receptor signaling pathway, TNF signaling pathway, Neurotrophin signaling pathway, GnRH signaling pathway, Estrogen signaling pathway, Oxytocin signaling pathway, Relaxin signaling pathway, Non-alcoholic fatty liver disease, AGE-RAGE signaling pathway in diabetic complications, Cocaine addiction, Amphetamine addiction, Epithelial cell signaling in Helicobacter pylori infection, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection, Pertussis, Yersinia infection, Leishmaniasis, Chagas di

Function: Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'
PTM: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC
Similarity: Belongs to the bZIP family
Similarity: Belongs to the bZIP family. Jun subfamily
Similarity: Contains 1 bZIP domain
Subunit: Heterodimer with either FOS or BATF3. Interacts with HIVEP3 (By similarity). Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5, indirectly leading to its phosphorylation. Interacts with DSIPI, this interaction inhibits the binding of active AP1 to its target DNA

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: JUN

Protein Name: Transcription factor AP-1, jun, c-jun；AP-1

Mouse Gene ID: 16476

Mouse SwissProt Number: P05627

Rat Gene ID: 24516

Rat SwissProt Number: P17325

Research Use Only