Product Description

AP2B1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES18309-50, ES18309-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human AP2B1 AA range: 70-120

Isotype: IgG

Target: AP2B1

Specificity: This antibody detects endogenous levels of AP2B1 at Human/Mouse/Rat

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in the brain (at protein level),

Molecular Weight (Da): 103kD

Fields: Endocytosis, Synaptic vesicle cycle, Endocrine and other factor-regulated calcium reabsorption, Huntington disease

Function: Function: Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP2B1 is a subunit of the plasma membrane adaptor. The complex binds polyphosphoinositides
Similarity: Belongs to the adaptor complexes large subunit family
Subcellular Location: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane
Subunit: Adaptor protein complex 2 (AP-2) is an heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Binds EPN1, EPS15, SNAP91 and clathrin

Subcellular Location: Cell membrane Membrane, coated pit, Peripheral membrane protein, Cytoplasmic side AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV

Storage and Stability: -20°C/1 year

Gene Name: AP2B1 ADTB2 CLAPB1

Protein Name: AP2B1

Mouse Gene ID: 71770

Mouse SwissProt Number: Q9DBG3

Rat Gene ID: 100912146

Rat SwissProt Number: P62944

Research Use Only