Product Description

Apaf-1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4108-50, ES4108-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: APAF1, KIAA0413, Apoptotic protease-activating factor 1, APAF-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550

Isotype: IgG

Target: APAF1

Specificity: Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous, Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung, Isoform 1 is expressed in heart, kidney and liver,

Observed Band (KD): 135kD

Fields: Platinum drug resistance, p53 signaling pathway, Apoptosis, Apoptosis - multiple species, Alzheimer disease, Parkinson disease, Amyotrophic lateral sclerosis, Huntington disease, Prion disease, Pathways of neurodegeneration - multiple diseases, Legionellosis, Tuberculosis, Hepatitis C, Hepatitis B, Measles, Influenza A, Herpes simplex virus 1 infection, Epstein-Barr virus infection, Pathways in cancer, Small cell lung cancer, Lipid and atherosclerosis

Function: Domain: The CARD domain mediates interaction with APIP
Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis
Induction: By E2F and p53 in apoptotic neurons
Similarity: Contains 1 CARD domain
Similarity: Contains 1 NB-ARC domain
Similarity: Contains 13 WD repeats
Subunit: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP
Tissue Specificity: Ubiquitous. Highest levels of expression in adult spleen and per

Subcellular Location: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: APAF1

Protein Name: Apoptotic protease-activating factor 1

Mouse Gene ID: 11783

Mouse SwissProt Number: O88879

Rat Gene ID: 78963

Rat SwissProt Number: Q9EPV5

Research Use Only