Product Description

APEX2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES18299-50, ES18299-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human APEX2 AA range: 196-246

Isotype: IgG

Target: APEX2

Specificity: This antibody detects endogenous levels of APEX2 at Human/Mouse

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Highly expressed in brain and kidney, Weakly expressed in the fetal brain,

Molecular Weight (Da): 57kD

Fields: Base excision repair

Function: Catalytic Activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Function: May participate in both nuclear and mitochondrial post-replicative base excision repair (BER). In the nucleus functions in the PCNA-dependent BER pathway
Similarity: Belongs to the DNA repair enzymes AP/exoA family
Subcellular Location: Colocalized partly with PCNA in nuclear foci
Subunit: Interacts with PCNA. This interaction is increased by misincorporation of uracil in nuclear DNA
Tissue Specificity: Highly expressed in cells, adult brain and kidney. Weakly expressed in the fetal brain

Subcellular Location: Nucleus Cytoplasm Mitochondrion Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents

Storage and Stability: -20°C/1 year

Gene Name: APEX2 APE2 APEXL2 XTH2

Protein Name: APEX2

Mouse Gene ID: 77622

Mouse SwissProt Number: Q68G58

Research Use Only