Product Description

AQP4 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1689-50, ES1689-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: AQP4, Aquaporin-4, AQP-4, Mercurial-insensitive water channel, MIWC, WCH4

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human AQP4. AA range:204-253

Isotype: IgG

Target: AQP4

Specificity: AQP4 Polyclonal Antibody detects endogenous levels of AQP4 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in skeletal muscle (PubMed:29055082), Detected in stomach, along the glandular base region of the fundic gland (at protein level) (PubMed:8601457), Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary (PubMed:7559426, PubMed:8601457),

Observed Band (KD): 35kD

Fields: Vasopressin-regulated water reabsorption, Bile secretion

Function: Domain: Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA)
Function: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system
Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family
Tissue Specificity: Brain - muscle heart, kidney, lung, and trachea

Subcellular Location: Cell membrane, Multi-pass membrane protein Basolateral cell membrane, Multi-pass membrane protein Endosome membrane Cell membrane, sarcolemma, Multi-pass membrane protein Cell projection Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane Detected on brain astrocyte processes and astrocyte endfeet close to capillaries

Storage and Stability: -20°C/1 year

Gene Name: AQP4

Protein Name: Aquaporin-4

Mouse Gene ID: 11829

Mouse SwissProt Number: P55088

Rat Gene ID: 25293

Rat SwissProt Number: P47863

Research Use Only