Product Description

ARA70 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1692-50, ES1692-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, CoIP, ELISA

Alternate Names: NCOA4, ARA70, ELE1, RFG, Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from ARA70. at AA range: 320-400

Isotype: IgG

Target: ARA70

Specificity: ARA70 Polyclonal Antibody detects endogenous levels of ARA70 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. IP 1:50-200Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed, Also detected in adipose tissues and in different cell lines, Isoform Beta is only expressed in testis,

Observed Band (KD): 70kD

Fields: Ferroptosis, Pathways in cancer, Thyroid cancer

Function: Alternative Products: Additional isoforms seem to exist,Disease: A chromosomal aberration involving NCOA4 is a cause of thyroid papillary carcinoma (PACT) [MIM: 188550]. Inversion inv(10)(q11.2, q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas
Function: Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma
polymorphism: Polymorphisms are specific to isoform alpha. Not found in isoform beta. Could be encoded by two different genes
Subunit: Interacts with the androgen receptor and the retinoid X receptor (RXR) in a ligand-dependent manner
Tissue Specificity: Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform beta is only expressed in testis

Subcellular Location: nucleus, autolysosome,

Storage and Stability: -20°C/1 year

Gene Name: NCOA4

Protein Name: Nuclear receptor coactivator 4

Research Use Only