Product Description

Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K236-M-48, E-BC-K236-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Abbreviation: AST

Target Synonym: AST;GOT;Glutamic-oxalacetic transaminase

Research Areas: Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 1.10-72.30 IU/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell;Cell culture supernatant

Assay Length: 1 h 20 min

Sensitivity: 1.10 IU/L

Inter cv: 6.8%

Intra cv: 5.3%

Research Use Only