Product Description

Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit (Aspartate Substrate Method)

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K1301-M-48, E-BC-K1301-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Glutamic-oxaloacetic transaminase (GOT), also known as aspartate aminotransferase (AST), is an important indicator reflecting liver function. AST in the sample catalyzes the deamination of L-aspartic acid to produce oxaloacetic acid. Oxaloacetic acid is reduced by the enzyme while NADH is oxidized to NAD+, resulting in a decrease in the absorbance at 340 nm. By monitoring the rate of the decrease in absorbance at 340 nm, the enzymatic activity of AST can be determined.

Research Areas: Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 5.81-1161.13 U/L

Tested Sample Types: Animal tissue;Cell;Serum;Plasma

Assay Length: 30 min

Sensitivity: 5.81 U/L

Inter cv: 2.1%-5.8%

Intra cv: 2.6%-4.8%

Research Use Only