Product Description

ATG4A Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8928-50, ES8928-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: Cysteine protease ATG4A (EC 3.4.22.-) (AUT-like 2 cysteine endopeptidase) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (Autophagy-related protein 4 homolog A) (hAPG4A)

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human ATG4A Polyclonal

Isotype: IgG

Target: ATG4A

Specificity: This antibody detects endogenous levels of ATG4A.

Dilution: WB 1:500-2000, ELISA 1:10000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Epithelium, Kidney, Ovary, Prostate, Testis,

Observed Band (KD): 40kD

Fields: Autophagy - other, Autophagy - animal

Function: Enzyme Regulation: Inhibited by N-ethylmaleimide
Function: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP
Similarity: Belongs to the peptidase C54 family
Tissue Specificity: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver

Subcellular Location: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: ATG4A APG4A AUTL2

Protein Name: Cysteine protease ATG4A (EC 3.4.22.-) (AUT-like 2 cysteine endopeptidase) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (Autophagy-related protein 4 homolog A) (hAPG4A)

Mouse Gene ID: 666468

Mouse SwissProt Number: Q8C9S8

Research Use Only